Puerto Madryn is a town in Chubut Province, Patagonia, Argentina.

The town was founded on July 28, 1865. 150 Welsh settlers of the ship The Mimosa named the natural port Puerto Madryn after Loves Jones Parry, who was Madryn Baron in Welsh. The settlement grew as a result of the railway track laid between Madryn and Trelew built by Welsh, Spanish, and Italian immigrants.

Puerto Madryn is protected by the Golfo Nuevo, which is formed by Peninsula Valdés and Punta Ninfas . It is important for visiting the natural attractions of the Valdes Peninsula and coast.