Nebraska was admitted to the Union on March 1, 1867.
Class 1
| Senator | Party | From
| To |
| John M. Thayer | Republican | 1867 | 1871 |
| Algernon S. Paddock | Republican | 1875 | 1881 |
| Charles H. Van Wyck | Republican | 1881 | 1887 |
| Algernon S. Paddock | Republican | 1887 | 1893 |
| William V. Allen | Populist | 1893 | 1899 |
| Monroe L. Hayward | Republican | 1899 | 1899 |
| William V. Allen | Populist | 1899 | 1901 |
| Charles H. Dietrich | Republican | 1901 | 1905 |
| Elmer J. Burkett | Republican | 1905 | 1911 |
| Gilbert M. Hitchcock | Democrat | 1911 | 1923 |
| Robert B. Howell | Republican | 1923 | 1933 |
| William H. Thompson | Democrat | 1933 | 1934 |
| Richard C. Hunter | Democrat | 1934 | 1935 |
| Edward R. Burke | Democrat | 1935 | 1941 |
| Hugh A. Butler | Republican | 1941 | 1954 |
| Samuel W. Reynolds | Republican | 1954 | |
| Roman L. Hruska | Republican | 1954 | 1976 |
| Edward Zorinsky | Democrat | 1976 | 1987 |
| David K. Karnes | Republican | 1987 | 1989 |
| Bob Kerrey | Democrat | 1989 | 2001 |
| Ben Nelson | Democrat | 2001 | |
Class 2
| Senator | Party | From
| To |
| Thomas W. Tipton | Republican | 1867 | 1875 |
| Phineas W. Hitchcock | Republican | 1871 | 1877 |
| Alvin Saunders | Republican | 1877 | 1883 |
| Charles F. Manderson | Republican | 1883 | 1895 |
| John M. Thurston | Republican | 1895 | 1901 |
| Joseph H. Millard | Republican | 1901 | 1907 |
| Norris Brown | Republican | 1907 | 1913 |
| George W. Norris | Republican,
Independent | 1913 | 1943 |
| Kenneth S. Wherry | Republican | 1943 | 1951 |
| Fred Andrew Seaton | Republican | 1951 | 1952 |
| Dwight P. Griswold | Republican | 1952 | 1954 |
| Eva Bowring | Republican | 1954 | |
| Hazel H. Abel | Republican | 1954 | |
| Carl T. Curtis | Republican | 1955 | 1979 |
| J. James Exon | Democrat | 1979 | 1997 |
| Chuck Hagel | Republican | 1997 | |
