Only current railroads are listed here.

See also List of defunct Florida railroads and List of Florida streetcar companies (all defunct).

Common carriers

• Alabama and Gulf Coast Railway (AGR) [1]
• Amtrak (AMTK) [2] (federally owned by the National Railroad Passenger Corporation)
• AN Railway (AN) [3]
• Bay Line Railroad (BAYL) [4]
• CSX Transportation (CSXT) [5] (Jacksonville and Florida Divisions, and one line of the Atlanta Division)
• Florida Central Railroad (FCEN) [6]
• Florida East Coast Railway (FEC) [7]
• Florida Midland Railroad (FMID) [8]
• Florida Northern Railroad (FNOR) [9]
• Florida West Coast Railroad (FWCR)
• Georgia and Florida RailNet (GFRR)
• Norfolk Southern Railway (NS) [10]
• Seminole Gulf Railway (SGLR) [11]
• South Central Florida Express (SCFE) [12]
• Talleyrand Terminal Railroad (TTR) [13]

Commuter railroads

• Jacksonville Transportation Authority (Skyway) [14]
• TECO Line Streetcar System (run by Hillsborough Area Regional Transportation Authority , managed by Tampa Historic Streetcar, Inc. ) [15]
• Miami-Dade Transit (Metromover and Metrorail) [16]
• South Florida Regional Transportation Authority (Tri-Rail) [17]