Interstate 395 is the designation for several Interstate Highways in the United States, all of which are related to Interstate 95:

• Interstate 395 (Connecticut) (also in Massachusetts), a spur to Worcester, Massachusetts
• Interstate 395 (Florida), a spur in Miami, Florida
• Interstate 395 (Maine), a spur in Bangor, Maine
• Interstate 395 (Maryland), a spur in Baltimore, Maryland
• Interstate 395 (District of Columbia/Virginia), a spur to Washington, D.C.

The following roads used to be numbered I-395:

• Interstate 676 (which has since been swapped with I-76), a loop through Philadelphia, Pennsylvania