Genesee and Wyoming, Inc., based in Greenwich, Connecticut, is a holding company of these short line railroads:
- Buffalo and Pittsburgh Railroad
- Empresa Ferroviaria Oriental
- Ferrocarriles Chiapas-Mayab
- Illinois and Midland Railroad
- Portland and Western Railroad
- Rochester and Southern Railroad
- St. Lawrence and Atlantic Railroad
- South Buffalo Railway
- Tazewell and Peoria Railroad
- Utah Railway
Through their subsidiary Genesee and Wyoming Canada, Inc., based in Montreal, Quebec:
Through their subsidiary GWI Australia Pty. Ltd., based in Welshpool, Western Australia :
- Australia Northern Railroad
- Australia Southern Railroad
- Australia Western Railroad (50% ownership)
- WestNet Rail (50% ownership)
Through their subsidiary Rail Link, Inc., based in Jacksonville, Florida:
- Carolina Coastal Railway
- Commonwealth Railway
- Corpus Christi Terminal Railroad
- Golden Isles Terminal Railroad
- Louisiana and Delta Railroad
- Savannah Port Terminal Railroad
- Talleyrand Terminal Railroad
- York Railway
External links