Dunfermline Palace is a former Scottish royal palace in Dunfermline, Fife.

The Palace of Dunfermline stood next to Dunfermline Abbey, occupying a picturesque position next to the ravine. It was rebuilt by James IV in 1500 and was a favourite residence of Scottish monarchs. James IV, James V, Mary, Queen of Scots and James VI all spent much of their time here. The palace was given as a wedding present to Anne of Denmark after her marriage to James VI in 1589. Prior to the Union of the Crowns in 1603 Anne of Denmark often stayed at the palace, and she gave birth to three of her children here; Elizabeth, Robert and Charles I in 1600. David II and James I of Scotland were also born at Dunfermline.

After Charles I's Scottish coronation in 1633 he paid a short visit to his birthplace. The last monarch to occupy the palace was Charles II who stayed at Dunfermline just before the Battle of Pitreavie in 1650. It was abandoned soon afterwards, and unroofed by 1708. All that is now left of the palace is the south wall and the kitchen.